Monitoring amyloid-β 42 conformational change using a spray-printed graphene electrode

Up to now, the reproducibility and stability of graphene-based electrochemical sensors have represented an obstacle development practical biosensing techniques. In this paper we report a cost-effective highly reproducible sensing platform monitor kinetic conformational change amyloidogenic proteins. The sensor surface is spray-printed with graphene oxide layer then electrochemically reduced achieve excellent sensitivity redox current. these in terms peak position, intensity electroactive area has been proved be high. These are used changes amyloid-β 42 via oxidation current tyrosine, which caused by different accessibility during aggregation process. process detected at shows good correlation fluorescence assay. proposed provides complementary technique aid understanding detailed protein mechanism neurodegenerative diseases as well helping promote disease-prevention strategies.